Tag Archive: SEM


Finally imaging with the microscope!

Loading the stubs part 2!

Loading the stubs into the sputter machine

The samples on the stubs.

The samples on the stubs.

The stubs in the sputter coating machine.

The stubs in the sputter coating machine.

Ever wonder what those numbers mean at the bottom of the SEM image? Here are some details on those numbers mean…

Vacc. = Vacuum, for biological samples 5kv is recommended.

W.D.= working distance, 12 to 15 mm is ideal..

magnification= how zoomed in you want your image to be…

date= date this image was taken

The image labels...

The image labels…

We are using the Hitachi S-2600N Variable Pressure Scanning Electron Microscope (VPSEM). Below are some pictures….

The scanning electron microscope.

The computers hooked up to the microscope.

The computers hooked up to the microscope.

The switches on the microscope.

The switches on the microscope.

The knobs for adjusting the images produced by the microscope.

The knobs for adjusting the images produced by the microscope.

There are some basic steps:

  1. collect samples
  2. dry your samples
  3. mount your samples on stubs with double sided glue stickers
  4. sputter coat your specimen with gold (the most commonly used)
  5. mount your specimen
  6. start SEM!

The following are some quick videos we took while we were trained on SEM. Perhaps, you might find it useful to view them, so you can grasp a quick understanding of the procedures. Just click on the underlined titles… it should bring you to the video pages…

On loading stubs into the sputter coating machine

On why we use gold for our specimens

On sputter coating

On turning off the sputter coating machine

On turning on the microscope and computer....

On loading the stubs into the microscope….

On loading the stubs into the microscope part 2

On working with the microscope

Loading the stubs into the microscope